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Progress in NMR-based metabolomics of

Qifang PAN,Jingya ZHAO,Yuliang WANG,Kexuan TANG

《农业科学与工程前沿（英文）》 2015年第2卷第3期页码 195-204 doi: 10.15302/J-FASE-2015063

摘要： Metabolomics has been rapidly developed as an important field in plant sciences and natural products chemistry. As the only natural source for a diversity of monoterpenoid indole alkaloids (MIAs), especially the low-abundance antitumor agents vinblastine and vincristine, is highly valued and has been studied extensively as a model for medicinal plants improvement. Due to multistep enzymatic biosynthesis and complex regulation, genetic modification in the MIA pathway has resulted in complicated changes of both secondary and primary metabolism in , affecting not only the MIA pathway but also other pathways. Research at the metabolic level is necessary to increase knowledge on the genetic regulation of the whole metabolic network connected to MIA biosynthesis. Nuclear magnetic resonance (NMR) is a very suitable and powerful complementary technique for the identiﬁcation and quantiﬁcation of metabolites in the plant matrix. NMR-based metabolomics has been used in studies of for pathway elucidation, understanding stress responses, classification among different cultivars, safety and quality controls of transgenic plants, cross talk between pathways, and diversion of carbon fluxes, with the aim of fully unravelling MIA biosynthesis, its regulation and the function of the alkaloids in the plant from a systems biology point of view.

关键词： Catharanthus roseus monoterpenoid indole alkaloids NMR metabolomics

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Biosynthetic pathway of terpenoid indole alkaloids in Catharanthus roseus

null

《医学前沿（英文）》 2014年第8卷第3期页码 285-293 doi: 10.1007/s11684-014-0350-2

摘要：

Catharanthus roseus is one of the most extensively investigated medicinal plants, which can produce more than 130 alkaloids, including the powerful antitumor drugs vinblastine and vincristine. Here we review the recent advances in the biosynthetic pathway of terpenoid indole alkaloids (TIAs) in C. roseus, and the identification and characterization of the corresponding enzymes involved in this pathway. Strictosidine is the central intermediate in the biosynthesis of different TIAs, which is formed by the condensation of secologanin and tryptamine. Secologanin is derived from terpenoid (isoprenoid) biosynthetic pathway, while tryptamine is derived from indole biosynthetic pathway. Then various specific end products are produced by different routes during downstream process. Although many genes and corresponding enzymes have been characterized in this pathway, our knowledge on the whole TIA biosynthetic pathway still remains largely unknown up to date. Full elucidation of TIA biosynthetic pathway is an important prerequisite to understand the regulation of the TIA biosynthesis in the medicinal plant and to produce valuable TIAs by synthetic biological technology.

关键词： Catharanthus roseus terpenoidindole alkaloids biosynthetic pathway vinblastine vincristine

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Promotive effect of pyridine on indole degradation by activated sludge under anoxic conditions

LI Yongmei, GU Guowei, LI Wenshu

《环境科学与工程前沿（英文）》 2007年第1卷第4期页码 493-497 doi: 10.1007/s11783-007-0079-0

摘要： Batch experiments were carried out to investigate the promotive effect of pyridine on indole degradation under denitrifying conditions. The seed sludge was obtained from a local coal-coking wastewater treatment facility and was acc

关键词： wastewater treatment coal-coking wastewater promotive degradation

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单萜类化合物芳樟醇、甲基丁香酚、草蒿脑和香茅醛对配体门控离子通道的作用模式 Article

Amy S. Li, Akimasa Iijima, Junhao Huang, Qing X. Li, Yongli Chen

《工程（英文）》 2020年第6卷第5期页码 541-545 doi: 10.1016/j.eng.2019.07.027

摘要：

在传统的医学治疗中，精油被用作镇静剂、抗惊厥剂以及局部麻醉剂，还可以用作储藏食品、水果、蔬菜和谷物等的防腐剂，同时，它还能用作食品生产的生物农药。芳樟醇（LL）以及其他一些主要组分，如甲基丁香酚（ME）、草蒿脑（EG）和香茅醛是许多精油（如罗勒精油）中的活性化学成分。罗勒精油以及上述单萜类化合物在驱除害虫等方面有明显的功效。然而，这些化学成分的作用机制尚不明确。众所周知，γ-氨基丁酸A型受体（GABAAR）和烟碱型乙酰胆碱受体（nAChR）是目前市场上使用的合成杀虫剂的主要分子靶标。此外，GABAAR靶向治疗也已经有了数十年的临床应用实践，如巴比妥类药物和苯二氮类药物。在本研究中，我们研究了LL、ME、EG和香茅醛对GABAAR和nAChR的电生理作用，以进一步了解它们作为传统药物治疗剂和杀虫剂的多功能性。研究结果表明，LL对GABAAR和nAChR均有抑制作用，这或许可以解释LL的杀虫活性。LL是GABAAR的一种浓度依赖性、非竞争性抑制剂，因为在实验中我们发现，小鼠α1β3γ2L GABAAR的γ-氨基丁酸（GABA）半最大效应浓度（EC50）值不受LL影响：在LL不存在或存在5 mmol·L^–1 LL的情况下，该半最大效应浓度值分别为(36.2 ± 7.9) μmol·L^–1以及(36.1 ± 23.8) μmol·L^–1。LL对GABAAR的半抑制浓度（IC50）约为3.2 mmol·L^–1。考虑到在精油之中存在着多种单萜类化合物，所以LL可能与此前被表征为GABAAR激动剂和正变构调节物的ME以及其他单萜类化合物具有协同作用，这为LL的镇静、抗惊厥效用和杀虫活性提供了一种可能的解释。

关键词：精油 γ-氨基丁酸A型受体芳樟醇单萜烟碱型乙酰胆碱受体